P-435 HPV and infertility in couples requiring Assisted Reproduction Techniques (ART).

Abstract Study question How frequent is Human Papilloma Virus (HPV) infection in both partners of infertile couples referring to our ART center and whats its impact? Summary answer Only 6% are positive for HPV has no impact on embryologic parameters. What known already There limited conflicting evidence about the relationship between male infertility. Notably, most previous studies did not assess presence context ART. Furthermore, even if considered, influence female partner may be underestimated because only data adverse pregnancy outcomes were evaluated. Elucidating HPV-positive status two individuals belonging couple could open up new scenarios. design, size, duration This prospective cohort study was performed 81 couples. The analysis conducted semen samples patients cervical who underwent assisted reproduction techniques (ART) treatment at infertility unit Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico Milan from February 2022 November 2022. Participants/materials, setting, methods partecipants recruited before beginning controlled ovarian stimulation (COS) while participants day ovum pick-up (OPU). technique used virus Real Time PCR (RT-PCR). Personal characteristics, lifestyle habits sexual investigated as possible favoring elements insurgence infection. Main results role chance Among recruited, 12 men 22 women tested high- low risk viruses. streins corrisponds a prevalence 15% (95% CI: 9-24%) males 27% 19-38%) females. these couples, 5 cases (6%, 95% 3-14%) simultaneously partners: pairs they same viral strains, other there concordance strains found, one pair partial concordance. McNemar's test (a dichotomous evaluation paired aimed assessing associations) significant. No differences embryo developmental failure, “top-quality” formation blastocyst found groups (positive vs negative couples). Limitations, reasons caution A limitation small sample size. Additionally we mainly focused by embryological variables, without considering pregnancies live birth rate. Wider implications findings very observed suggests that follows intricate pathways, far beyond trivial view ping-pong effect couple. These suggest probably develops independently couple, mutual influence. Trial registration number NOT APPLICABLE